The Dow Jones Industrial Average fell 234.21 points, or 0.6%, ending at 38,763.45.
The S&P 500 shed 40.53 points, or 0.8%, closing at 5,199.50.
The Nasdaq Composite dropped 171.05 points, or 1.1%, finishing at 16,195.81.
It has been the worst five-day start to a month for both the Dow and the S&P 500 since January 2016, according to Dow Jones Market Data.
The selloff in U.S. equities resumed, despite a sharp rebound for Japanese stocks, with the Nikkei 225 up 1.2% on Wednesday.
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